Ddr1 assay
WebPathHunter® DDR1 Functional Assay. PathHunter® RTK Functional cell lines are engineered to co-express a ProLink™ (PK) tagged RTK, an Enzyme Acceptor (EA) tagged SH2 domain, and, in some cases, an … WebJun 28, 2024 · b: Colony formation assay after knockdown of DDR1 in OEC-M1 and TW2.6 cells for 10 days (left). The mean number of colonies for each well was determined from three independent assays (right). c: Growth rates of OEC-M1 and TW2.6 cells measured by MTT assay after DDR1 knockdown.
Ddr1 assay
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WebDiscovery of a broad spectrum antiproliferative agent with selectivity for DDR1 kinase: cell line-based assay, kinase panel, molecular docking, and toxicity studies Discovery of a broad spectrum antiproliferative agent with selectivity for DDR1 kinase: cell line-based assay, kinase panel, molecular docking, and toxicity studies WebNov 3, 2024 · Here we show that discoidin domain receptor 1 (DDR1), a collagen receptor with tyrosine kinase activity 5, instigates immune exclusion by promoting collagen fibre alignment. Ablation of Ddr1 in ...
WebAssay Measures. Receptor Phosphorylation. How it works. Cells were plated in a 96-well plate and incubated for 24 or 48 hours at 37°C/5% CO2. Cells were then stimulated with a control agonist. Following stimulation, … WebLanthaScreen® Eu Kinase Binding Assay for DDR1 Overview This protocol describes how to perform a LanthaScreen® Eu Kinase Bindng Assay designed to detect and …
WebTo further investigate the effects of DDR1 on tumor cell growth in vivo, a tumorigenesis assay was performed in BALB/c female mice using 4T1 cells with or without stable DDR1 overexpression. The tumors in the … WebDiscoidin domain receptor family, member 1 (DDR1), also known as or CD167a (cluster of differentiation 167a), and Mammary carcinoma kinase 10 (MCK10), belongs to a subfamily of tyrosine kinase receptors with an extracellular domain homologous to Dictyostellium discoideum protein discoidin 1.
WebFeb 9, 2024 · To examine whether the interaction depends on DDR1 kinase activity, we introduced DDR1-K618A for Co-IP assay. The results suggested that the interaction …
WebDec 31, 2024 · After DEL bead encapsulation in DDR1 binding assay droplets and UV dosing to cleave the library member from the bead, the droplets flow through the incubator (~12 min), and pass through the laser focus for detection (6000 Hz) of FP signal. Droplets exhibiting FP <4σ below the mean FP of unoccupied droplets were sorted as hits (Figure … c# list with objectsWebAssay Format. HEK293 cells transiently expressing NanoLuc-DDR1 Fusion Vector were seeded into the wells of 384-well plates. The cells were pre-treated with the NanoBRET … c# list where multiple conditionsWebDDR1 is known to interact with other receptors (Integrin-R and Notch-R) and some of the effector functions are due to these interactions. As shown the signaling events enhance the expression of... c# list with 2 typesWebSep 2, 2024 · Next, we investigated the DDR1 inhibitory activity of compound 1 and compound 2 as measured by autophosphorylation in U2OS cells. The compounds showed IC 50 values of 10.3 and 5.8 nM,... c list with multiple typesWebThis study reports the identification of a potent DDR1 inhibitor, nilotinib, for the treatment of breast cancer. MTT assay was used to evaluate the inhibitory activity of nilotinib and meantime we used flow cytometry to evaluate the pro-apoptotic activity of nilotinib in MCF-7 and MDA-MB-231 cells. c# list where in listWebDDR1-IN-1 is a potent and selective discoidin domain receptor 1 (DDR1) receptor tyrosine kinase inhibitor with IC50 of 105 nM, about 3-fold selectivity over DDR2. CAS No. … bob \u0026 tom show alli breenWebSep 21, 2024 · The Discoidin Domain Receptor 1 (DDR1) is one of the two members of a unique family of receptor tyrosine kinase receptors that signal in response to collagen, … bob \u0026 sons insurance portland in