WebExamples include carminic acid, celestine blue, gallocyanine, hematein and nuclear fast red. Some are used to generate colored products from tissue constituents, e.g.: … WebFluorochrome staining: The use of any fluorescent dye (e.g., auramine, rhodamine) used to label or stain. Must be viewed using a fluorescence microscope. Ziehl-Neelsen or Kinyoun: Methods for staining acid-fast bacteria; acid-fast organisms appear red on a light blue or green background; carbol fuchsin is the primary stain for these methods.

Selecting Fluorochromes for Flow Cytometry - University …

WebFor example, propidium iodide (PI), which is used to stain DNA, is only weakly fluorescent in water; on intercalating with DNA, the fluorescence increases 50 fold due to the hydrophobic environment. Some … WebJun 13, 2024 · Some common examples for fluorophores are as follows: Xanthene derivatives such as fluorescein Cyanine Naphthalene derivatives Coumarin derivatives Pyrene derivatives such as cascade blue … reaction of detereum with benzen

Fluorochrome definition and meaning Collins English Dictionary

WebJan 10, 2024 · Tab. 1: This example of a multicolor indirect Immunofluorescence demonstrates how to label simultanously three different proteins in the same cell. The three first antibodies must derive from different species in order to detect them with three different fluorochrome-coupled secondary antibodies. WebCommonly used tandem fluorochromes used for flow cytometry such as PerCP-Cy5.5, PerCP-eFluor710, PE-Cy7, APC-Cy7, BV605, BV650, BV711, BV786, etc. vary in their … WebFor example, the tandem dye PE-Cy5 consists of phycoerythrin and a cyanine dye. In tandem dyes one of the fluorochromes is excited by the laser and is, in turn, able to transfer its fluorescent energy to the second molecule which then emits light of a different, longer wavelength. reaction of cyclohexene with bromine water