Klf5 chip

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August undefined, 2024

WebAug 31, 2024 · Klf5 regulates muscle differentiation via directly targeted muscle-specific genes in cooperation with MyoD in mice. Organism. Mus musculus. Experiment type. … WebSep 3, 2024 · This new KLF5 target gene programme has potential prognostic significance as high levels correlate with poorer patient survival. Thus, the repurposing of KLF5 for …

A stem cell marker KLF5 regulates - Nature

WebFeb 21, 2024 · Krüppel-like Factor 5 (KLF5) is a cell-intrinsic oncogene to promote tumorigenesis. However, the cell-extrinsic effects of KLF5 on suppressing the immune … WebAug 1, 2024 · KLF5 regulates key biological processes in the human airway epithelium To determine the target genes of KLF5 in human lung epithelial cells, we performed RNA-seq on triplicate samples of Calu-3 cells treated with either negative control (NC) siRNA or siRNA specific for KLF5. the brand edit

Regulatory role of Klf5 in early mouse development and in

WebApr 13, 2024 · ChIP-seq of B2B1 samples was successful when immunoprecipitating EGFR chimeras, ... NEAT1) and transcription factors of the AP1 (JUNB) and KLF (KLF5) families. WebFeb 10, 2008 · Interestingly, the ChIP-on-chip data revealed a weak signal of Klf2, Klf4 and Klf5 occupancies at the Fgf5 and Nes promoters. This binding was confirmed using ChIP–qPCR assays, and showed... WebWe performed RNA sequencing, immunohistochemistry, western blotting, real time-PCR, ELISA, luciferase assay, chromatin immunoprecipitation (ChIP), and flow cytometry to demonstrate the effects of KLF5 on CD8 + T cell infiltration and related molecular mechanism. Single-cell RNA sequencing and spatial transcriptomics analysis were … the brand digital media

A core Klf circuitry regulates self-renewal of embryonic stem cells

TP63, SOX2, and KLF5 Establish a Core Regulatory Circuitry That ...

WebOct 1, 2024 · TP63, SOX2, and KLF5 Establish a Core Regulatory Circuitry That Controls Epigenetic and Transcription Patterns in Esophageal Squamous Cell Carcinoma Cell Lines - ScienceDirect Gastroenterology Volume 159, Issue 4, October 2024, Pages 1311-1327.e19 Original Research Full Report: Basic and Translational—Alimentary Tract WebJan 13, 2024 · Mechanistically, YB-1 enhances KLF5 expression not only through transcriptional activation that can be inhibited by DACH1, but also by stabilizing KLF5 … the brand distilleryWebJan 24, 2024 · ChIP assays were conducted to verify the enrichment of the potential binding sites by KLF5 antibody in BT-549 cells. The ChIP results were analyzed by qPCR and nucleic acid electrophoresis. The dual-luciferase reporter assays were performed to validate the interaction between KLF5 and the first binding site (E1). the brand exact

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Klf5 chip

Inhibition of HDAC4 by GSK3β leads to downregulation of KLF5 …

WebMar 17, 2024 · ChIP with the KLF5 antibody from R&D (#AF3758) was performed using the SimpleChIP® Enzymatic Chromatin IP Kit (Cell Signaling, #9003) following the … WebIn epigenetic and transcriptional analyses of ESCC cell lines, we found the transcription factors TP63, SOX2, and KLF5 to be part of a core regulatory network that determines chromatin accessibility, epigenetic modifications, and gene expression patterns in these cells. A combination of epigenetic i …

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WebHIF-1α活化对肾小管间质炎症和纤维化的影响及其机制探讨.pdf WebOct 27, 2024 · Fig. 1: Identification of the KLF5 enhancer region by in vitro enChIP-seq. a List of regions that bind to the KLF5 promoter by in vitro enChIP-seq analysis. The regions of …

WebMar 21, 2024 · KLF5 (KLF Transcription Factor 5) is a Protein Coding gene. Diseases associated with KLF5 include Cardiomyopathy, Dilated, 1Ff and Sigmoid Disease.Among its related pathways are White fat cell differentiation and RNA Polymerase I Promoter Opening.Gene Ontology (GO) annotations related to this gene include DNA-binding … WebOverview. A cell-permeable phenylpropenoate compound that inhibits the proliferation of colon cancer cell lines exhibiting high levels of KLF5 (IC 50 = 1.6 µM in DLD-1 cells). …

WebAntibodies that detect KLF5 can be used in several scientific applications, including Western Blot, Immunocytochemistry, Immunohistochemistry, ChIP and ELISA. These antibodies target KLF5 in Human, Mouse and Rat samples. Our KLF5 polyclonal, monoclonal, recombinant monoclonal and recombinant polyclonal antibodies are developed in Rabbit … WebNov 9, 2024 · Klf5 is essential for bi-potential cell fate; a single Klf5-overexpressing embryonic stem cell (ESC) generates terminally differentiated embryonic and extra …

WebAntibodies that detect KLF5 can be used in several scientific applications, including Western Blot, Immunocytochemistry, Immunohistochemistry, ChIP and ELISA. These antibodies …

WebNov 9, 2024 · Although Klf5 has the ability to induce ICM and TE genes based on our ChIP-seq data, Klf5 mediates gene transcription in a cell-type- and context-specific manner. In pluripotent ESCs, Klf5 overexpression induced multiple pluripotency transcription factors, including Nanog, ... the brand educationWebKLF5 ChIP-seq on human GM12878 Nucleic acid type DNA Size range 450-650 Platform Illumina HiSeq 2000 Controls ENCSR398JTO Attribution Lab Michael Snyder, Stanford Award U54HG006996 (Michael Snyder, Stanford) Project ENCODE External resources GEO:GSE127670 References PMID:31969180 PMCID:PMC7333332 PMCID:PMC6724667 … the brand energy modelWebMar 17, 2024 · ChIP with the KLF5 antibody from R&D (#AF3758) was performed using the SimpleChIP® Enzymatic Chromatin IP Kit (Cell Signaling, #9003) following the manufacture’s instructions. Primers for these analyses are listed in Supplementary Table 3. The SYBR green (Takara, Mountain View, CA) method was used with the 7500 Fast Real … the brand exchange pty ltdWebOct 28, 2024 · Klf5 is an essential regulator of cell fate during early embryonic development, and it can also be used to generate pluripotent stem cells and to maintain their potency and self-renewal... the brand establishmentWebFeb 20, 2024 · ChIP-seq data Rogerson et al., 2024 demonstrate that KLF5 strongly binds to the JUP and CCNE1 enhancers but low levels are observed at the MYBL2 enhancer . KLF5 depletion ( Figure 3—figure supplement 1H ) caused reduced expression of all three target genes ( Figure 3H ). the brand ericaWebKLF5 is a Krüppel-like transcription factor broadly involved in development and regeneration whose lineage specificity, if any, remains unclear. Here we report KLF5 specifically marks the epidermis, and its deletion leads to skin barrier dysfunction in vivo. the brand equityWebMar 10, 2024 · The mRNA and protein expressions of KLF5 and ASK1 were increased in NP tissues of IDD mice when compared to control mice (Fig. 2 a, b). In addition, chromatin immunoprecipitation (ChIP)-PCR experimental results showed that HDAC4 overexpression significantly enriched HDAC4 in the KLF5 promoter region (Fig. 2 c). the brand elevate